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Traditional use of royal jelly in preventing aging has led to experiments regarding neuronal activities. Stimulation of production of glial cell line-derived neurotrophic factor has been demonstrated in the adult mouse brain, with a prediction of a neuroprotective role for royal jelly.  


In addition, 10-hydroxy-trans-2-decanoic acid increased the generation of neurons from neural stem (progenitor) cells in vitro,  while adenosine monophosphate stimulated neuronal differentiation of pheochromocytoma PC12 cells.  


Activity on the pituitary gland in middle-aged rats has also been demonstrated, and orally administered royal jelly increased granule cell content in the hippocampus, with an observed improvement in induced cognitive impairment in mice.